![]() Next to l-AAs, endogenous levels of d-glutamine and d-aspartic acid could be measured in CSF revealing enantiomeric ratios of 0.35 and 19.6%, respectively. The applicability of the method was investigated by the analysis of cerebrospinal fluid (CSF). Fluorescence detection was achieved employing optical fibers, leading UV excitation light ( 0.997) and repeatability for peak height (relative standard deviations (RSDs) < 7.0% n = 5) and electrophoretic mobility (RSDs < 0.6% n = 5) of individual AA enantiomers were established for chiral analysis of dl-AA mixtures. dl-AAs were derivatized with 9-fluorenylmethoxycarbonyl chloride (FMOC)-Cl to allow their fluorescence detection and enhance enantioseparation. The potential of capillary electrophoresis (CE) with ultraviolet (UV)-excited fluorescence detection for sensitive chiral analysis of amino acids (AAs) was investigated. ![]()
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